Three-Dimensional Visualization of Protein Expression in Mouse Brain Structures
Using Imaging Mass Spectrometry
Anna Crecelius, D. Shannon Cornett, Richard M. Caprioli, Betsy Williams, Benoit Dawant, and Bobby Bodenheimer
Abstract
We have developed a method to visualize matrix-assisted laser desorption ionization imaging
mass spectrometry (MALDI IMS) data aligned with optically determinable tissue structures in
three dimensions. Details of the methodology are exemplified using the 3-D reconstruction of
myelin basic protein (MBP) in the corpus callosum of a mouse brain. In this procedure, optical
images obtained from serial coronal sections are first aligned to each other to reconstruct a
surface of the corpus callosum from segmented contours of the aligned images. The MALDI
IMS data are then coregistered to the optical images and superimposed into the surface to
create the final 3-D visualization. Correlating proteomic data with anatomical structures
provides a more comprehensive understanding of healthy and pathological brain functions,
and holds promise to be utilized in more complex anatomical arrangements. (
J Am Soc Mass
Spectrom 2005, 16, 1093-1099). © 2005 American Society for Mass Spectrometry. DOI:10.1016/j.jasms.2005.02.026.
Bobby Bodenheimer 